1,906 research outputs found
Variabilité protéique observée par électrophorèse bidimensionnelle sur neuf isolats d'Heterodera schachtii provenant de six pays européens et sur deux isolats d'H. trifolii f.sp. beta
La variabilité protéique de neuf isolats européens d'#Heterodera schachtii et de deux isolats d'#H. trifolii f. sp. #beta élevés en conditions contrôlées sur colza, #Brassica napus L. var. #napus$ cv. Samouraï a été étudiée par électrophorèse bidimensionnelle (E2D). Les gels électrophorétiques sont comparés à l'aide d'un système informatique d'analyse d'images. Les indices de similarité (F) et les distances génétiques (D = 1-F) sont calculés à partir des taches homologues. Le dendrogramme est obtenu avec la méthode UPGMA. Les résultats montrent une assez grande similitude protéique des deux espèces. Les principales différences interspécifiques sont mises en évidence, ainsi que deux zones communes caractéristiques. Concernant la variabilité infraspécifique, des taches caratéristiques de certains isolats ont été repérées. La possibilité d'utiliser l'E2D pour identifier des protéines liées à la virulence est envisagée, ce qui nécessitera l'étude d'un grand nombre d'isolats. (Résumé d'auteur
A transient network of telechelic polymers and microspheres : structure and rheology
We study the structure and dynamics of a transient network composed of
droplets of microemulsion connected by telechelic polymers. The polymer induces
a bridging attraction between droplets without changing their shape. A
viscoelastic behaviour is induced in the initially liquid solution,
characterised in the linear regime by a stretched exponential stress
relaxation. We analyse this relaxation in the light of classical theories of
transient networks. The role of the elastic reorganisations in the deformed
network is emphasized. In the non linear regime, a fast relaxation dynamics is
followed by a second one having the same rate as in the linear regime. This
behaviour, under step strain experiments, should induce a non monotonic
behaviour in the elastic component of the stress under constant shear rate.
However, we obtain in this case a singularity in the flow curve very different
from the one observed in other systems, that we interpret in terms of fracture
behaviour.Comment: 9 pages, 4 figure
Casimir forces in modulated systems
For the first time we present analytical results for the contribution of
electromagnetic fluctuations into thermodynamic properties of modulated
systems, like cholesteric or smectic liquid crystalline films. In the case of
small dielectric anisotropy we have derived explicit analytical expressions for
the chemical potential of such systems. Two limiting cases were specifically
considered: (i) the Van der Waals (VdW) limit, i.e., in the case when the
retardation of the electromagnetic interactions can be neglected; and (ii) the
Casimir limit, i.e. when the effects of retardation becomes considerable. It
was shown that in the Casimir limit, the film chemical potential oscillates
with the thickness of the film. This non-monotonic dependence of the chemical
potential on the film thickness can lead to step-wise wetting phenomena,
surface anchoring reorientation and other important effects. Applications of
the results may concern the various systems in soft matter or condensed matter
physics with multilayer or modulated structures.Comment: 13 page
Time scales in shear banding of wormlike micelles
Transient stress and birefringence measurements are performed on wormlike micellar solutions that "shear band", i.e. undergo flow-induced coexistence of states of different viscosities along a constant stress "plateau". Three well-defined relaxation times are found after a strain rate step between two banded flow states on the stress plateau. Using the Johnson-Segalman model, we relate these time scales to three qualitatively different stages in the evolution of the bands and the interface between them: band destabilization, reconstruction of the interface, and travel of the fully formed interface. The longest timescale is then used to estimate the magnitude of the (unknown) "gradient" terms that must be added to constitutive relations to explain the history independence of the steady flow and the plateau stress selection
Entropic phase separation of linked beads
We study theoretically a model system of a transient network of microemulsion
droplets connected by telechelic polymers and explain recent experimental
findings. Despite the absence of any specific interactions between either the
droplets or polymer chains, we predict that as the number of polymers per drop
is increased, the system undergoes a first order phase separation into a dense,
highly connected phase, in equilibrium with dilute droplets, decorated by
polymer loops. The phase transition is purely entropic and is driven by the
interplay between the translational entropy of the drops and the
configurational entropy of the polymer connections between them. Because it is
dominated by entropic effects, the phase separation mechanism of the system is
extremely robust and does not depend on the particlular physical realization of
the network. The discussed model applies as well to other polymer linked
particle aggregates, such as nano-particles connected with short DNA linkers
To robotize chemistry laboratories. An example of organic synthesis: 2-Boc-amino-N-hydroxy-3-phenyl-propionamide
The paper describes the development of periodic modules used for the peptide synthesis of hydroxamic acid. A powder conveyor using the principle of positive weighing distribution is described. Purification is provided using automatic filtration and a liquid— liquid extraction module separation device. Device quality is improved using failure mode and effects analysis
Bebida contendo abacaxi (Ananas comosus) e beterraba (Beta vulgaris) para crianças: tratar termicamente ou não?
Edição dos Resumos do VI Congresso Latinoamericano e XII Congresso Brasileiro de Higienistas de Alimentos, II Encontro Nacional de Vigilância das Zoonoses, IV Encontro do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Gramado, abr. 2013
Population pharmacokinetics of lopinavir and ritonavir in combination with rifampicin-based antitubercular treatment in HIV-infected children
Children with HIV associated tuberculosis often require co-formulated lopinavir/ritonavir (LPV/RTV)-based antiretroviral treatment with rifampicin-based antitubercular treatment (ATT). Rifampicin (RIF), a potent inducer of drug-metabolizing systems, profoundly reduces the bioavailability of LPV. The aims of this study were to develop an integrated population pharmacokinetic (PK) model describing LPV and RTV PK in children with and without concomitant ATT using two different dosing approaches and to estimate doses of LPV/RTV achieving target exposures during ATT in young children
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